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KMID : 0363320030240010123
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Journal of Korean Oriental Internal Medicine
2003 Volume.24 No. 1 p.123 ~ p.133
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Effects of Chungganhaeju-tang on Gene Expression of Alcohol-metabolizing Enzymes and Alcohol-induced Apoptosis
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Kim Young-Tae
Kim Young-Chul
Lee Jang-Hoon
Woo Hong-Jung
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Abstract
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Objectives : This study was designed to investigate the effects of Chungganhaeju-tang on expression of alcohol metabolizing enzymes, cell viability and alcohol-induced apoptosis.
Materials and Methods : For this study, the human hepatoma cell line HepG2 was used. HepG2 cells were treated with ethanol-or acetaldehyde, chungganhaeju-tang, anti-Fas neutralizing antibody and were investigated by using quantitative RT-PCR, MTT and Trypan blue exclusion assays.
Results : The results are summarized as follows:
1. Quantitative RT-PCR analysis demonstrated that ethanol-or acetaldehyde-mediated increase of ALDH gene expression was not affected by Chungganhaeju-tang treatment.
2, Ethanol-or acetaldehyde-induced apoptosis was remarkably inhibited by Chungganhaeju-tang in a dose-dependent manner.
3, Ethanol-or acetaldehyde-induced apoptosis was significantly blocked by anti-FasL neutralizing antibody, suggesting apoptosis induced by alcohol might be mediated by FasL/Fas signaling pathway.
Conclusions : Taken all together, these results indicate that the FasL/Fas signaling plays a critical role in alcohol-induced apoptosis and Chungganhaeju-tang increases viability of liver cells by suppression of the FasL/Fas-mediated apoptosis-signaling pathway.
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KEYWORD
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Chungganhaeju-tang(Qingganjiejiu-tang), ALDH(aldehyde dehydrogenase), HepG2, Fas, apoptosis
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